Proteinase activity in soybean nodules
نویسندگان
چکیده
منابع مشابه
Asparagine biosynthesis in soybean nodules.
Asparagine biosynthesis in soybean (Glycine max [L.] Merr.) nodules has been difficult to demonstrate due to the poor conversion of suspected immediate precursors to asparagine and the instability of the key enzyme asparagine synthetase. The present study was designed to explore the effects of two ammonium assimilation inhibitors on the metabolism of (14)CO(2) to [(14)C]asparagine and to demons...
متن کاملAsparagine formation in soybean nodules.
(15)NH(4) (+) and [(15)N](amide)-glutamine externally supplied to detached nodules from soybean plants (cv. Tamanishiki) were incorporated within nodule tissues by vacuum infiltration and metabolized to various nitrogen compounds during 60 minutes of incubation time. In the case of (15)NH(4) (+) - feeding, the (15)N abundance ratio was highest in the amide nitrogen of glutamine, followed by glu...
متن کاملSucrose synthase of soybean nodules.
SUCROSE SYNTHASE (UDPGLUCOSE: d-fructose 2-alpha-d-glucosyl transferase, EC 2.4.1.13) has been purified from the plant cytosolic fraction of soybean (Glycine max L. Merr cv Williams) nodules. The native enzyme had a molecular weight of 400,000. The subunit molecular weight was 90,000 and a tetrameric structure is proposed for soybean nodule sucrose synthase. Optimum activity in the sucrose clea...
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Nodule extracts prepared from Glycine max var Woodworth possessed endopeptidase, aminoptidase, and carboxypeptidase actiities. Three distinct endopeptidase activities could be resolved by disc-gel electrophoresis at pH 8.8. According to their order of increasing electrophoretic mobility, the first of these enzymes hydrolyzed azocasein and n-benzoyl-LLeu-p8-naphthylamide, while the second hydrol...
متن کاملPropionate in heme biosynthesis in soybean nodules.
When soybean nodules are incubated with propionate-2-(14)C the heme moiety of leghemoglobin becomes labeled. The incorporation of propionate-2-(14)C into heme is linear with time and it appears that propionate is utilized without a lag period. The rate of incorporation of propionate-2-(14)C into heme is more rapid than the rate of incorporation of succinate-2-(14)C and citrate-1,5-(14)C, howeve...
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ژورنال
عنوان ژورنال: South African Journal of Botany
سال: 2008
ISSN: 0254-6299
DOI: 10.1016/j.sajb.2008.01.080